Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH10 polyclonal antibody (Cat # PAB3148) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of CEM cells using CDH10 polyclonal antibody (Cat # PAB3148)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis of CDH10 polyclonal antibody (Cat # PAB3148) in CEM cell line lysates (35 ug/lane). CDH10 (arrow) was detected using the purified polyclonal antibody.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of CDH10.PAB,polyclonal Ab,polyclonal antibody

    Immunogen

    A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CDH10.

    Host

    Rabbit

    Reactivity

    Human

    Form

    Liquid

    Purification

    Ammonium sulfate precipitation

    Recommend Usage

    Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH10 polyclonal antibody (Cat # PAB3148) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

    Flow Cytometry

    Flow cytometric analysis of CEM cells using CDH10 polyclonal antibody (Cat # PAB3148)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

    Western Blot (Cell lysate)

    Western blot analysis of CDH10 polyclonal antibody (Cat # PAB3148) in CEM cell line lysates (35 ug/lane). CDH10 (arrow) was detected using the purified polyclonal antibody.
  • Gene Info — CDH10

    Entrez GeneID

    1008

    Protein Accession#

    NP_006718;Q9Y6N8

    Gene Name

    CDH10

    Gene Alias

    -

    Gene Description

    cadherin 10, type 2 (T2-cadherin)

    Omim ID

    604555

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance. [provided by RefSeq

    Other Designations

    T2-cadherin|cadherin 10, type 2|cadherin-10

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