Images
Flow Cytometry
Application

Flow Cytometry

BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (FITC) (Cat # MAB5751). A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

  • Specifications

    Product Description

    Rat monoclonal antibody raised against native Cd86.MAB,monoclonal Ab,monoclonal antibody

    Immunogen

    Native purified Cd86 from LPS-activated mouse B cells.

    Host

    Rat

    Reactivity

    Mouse

    Specificity

    Mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4.

    Form

    Liquid

    Conjugation

    Biotin

    Isotype

    IgG2a, kappa

    Recommend Usage

    Flow Cytometry (1 ug/106 cells)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store in the dark at 4°C. Do not freeze.
    Avoid prolonged exposure to light.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Frozen sections)

    Flow Cytometry

    BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (FITC) (Cat # MAB5751). A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

    Immunoprecipitation

  • Gene Info — Cd86

    Entrez GeneID

    12524

    Gene Name

    Cd86

    Gene Alias

    B7, B7-2, B7.2, B70, CLS1, Cd28l2, ETC-1, Ly-58, Ly58, MB7, MB7-2, TS/A-2

    Gene Description

    CD86 antigen

    Gene Ontology

    Hyperlink

    Other Designations

    OTTMUSP00000015940|T-lymphocyte activation antigen CD86|activation B7-2 antigen|early T cell costimulatory molecule-1

  • Publication Reference
eProcurement