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> CD11b(M1/70), 0.2mg/mL

CD11b(M1/70), 0.2mg/mL

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Cat# BNUB0720-100

Size : 100uL

Brand : Biotium

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Phone : +1 850 650 7790

Antibody number

#0720
Antibody reactivity (target)

CD11b, MAC-1
Antibody type

Primary
Host species

Rat
Clonality

Monoclonal
Clone

M1/70
Isotype

IgG2b, kappa
Molecular weight

95 kDa & 170 kDa
Synonyms

CD11 antigen-like family member B, CD11b/CD18, CD49d, Cell surface glycoprotein MAC-1 subunit alpha, Complement Component Receptor 3 Alpha, CR3 Alpha Chain (CR3A), Integrin alpha-M, Integrin beta 2 alpha subunit, ITGAM, Leukocyte adhesion receptor MO1; Ly-40, Mac-1a, MAC1, Mac1, alpha subunit, MAC1A, Macrophage antigen alpha polypeptide; MO1A, Neutrophil adherence receptor alpha M subunit
Human gene symbol

ITGAM
Entrez gene ID

3684
SwissProt

P11215
Unigene

172631
Immunogen

B10 mouse spleen cells enriched for T lymphocytes
Antibody target cellular localization

Plasma membrane
Expected antibody applications

Flow, surface (published for clone), Functional studies (published for clone), IF (published for clone), IP (published for clone)
Species reactivity

Baboon, Chimpanzee, Human, Mouse, Rabbit, Rhesus monkey
Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL
Positive control

Monocytes & granulocytes. Lymph nodes and tonsils.
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas

Immunology, Neuroscience
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression

Microglia, Monocytes/macrophages, Neutrophils
Protocols

PI Protocols for Antibody Based Detection

SDS

SDS Primary Antibodies

Supporting Documents

CF-Dye-Brochure

CF® Dye Quick Reference Flyer

CF® Dyes Absorption and Emission Normalized Data

Download Datasheet

Product Description

CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an alpha chain and beta chain. IntegrinαM combines with the Integrinβ2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). IntegrinαM/β2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the

CF® Dye Brochure

for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email

info@quimigen.pt

to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0720, Anti-CD11b (M1/70) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405

CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC

CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC

CF®568 Features
BNC94 CF®594 593/614 561 Texas Red®

CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5

CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5

CF®647 Features
BNC74 CF®740 742/767 633-685 775/50

CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Note: References for this clone sold by other suppliers may be listed for expected applications.

  1. J Exp Med (1990) 171, 1753-1771. (IP)
  2. J Applied Physiol (2003) 95(3): 969-976. (functional studies)
  3. J Leukocyte Biol (2008) 83: 728 -741. (IF)
  4. Meth Enzymol (2014) 537: 297-314. (Flow)