Immunofluorescence
Immunofluorescent staining of CBX1 in baby hamster kidney cells with CBX1 polyclonal antibody (Cat # PAB15581). Cells were fixed with para-formaldehyde. The second antibody was Alexa Fluor 594 conjugated goat anti-rabbit IgG.
Western Blot (Cell lysate)
Western blot of MCF-7 cell extracts with CBX1 polyclonal antibody (Cat # PAB15581) at 1000 fold dilution.
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CBX1.
Immunogen
A synthetic peptide corresponding to amino acids 176-185 of human CBX1.
Sequence
NEDDDKKDDKN
Host
Rabbit
Reactivity
Hamster, Human
Form
Liquid
Recommend Usage
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 120 mM sodium phosphate buffer, pH 7.4 (50% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Immunofluorescence
Immunofluorescent staining of CBX1 in baby hamster kidney cells with CBX1 polyclonal antibody (Cat # PAB15581). Cells were fixed with para-formaldehyde. The second antibody was Alexa Fluor 594 conjugated goat anti-rabbit IgG.ChIP
Western Blot (Cell lysate)
Western blot of MCF-7 cell extracts with CBX1 polyclonal antibody (Cat # PAB15581) at 1000 fold dilution. - Gene Info — CBX1
Entrez GeneID
10951Gene Name
CBX1
Gene Alias
CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, M31, MOD1, p25beta
Gene Description
chromobox homolog 1 (HP1 beta homolog Drosophila )
Omim ID
604511Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
heterochromatin protein 1-beta|heterochromatin protein p25 beta|modifier 1 protein
- Interactomes
- Publication Reference
- The Heterochromatin Protein 1 family.
Lomberk G, Wallrath L, Urrutia R.
Genome Biology 2006 Jul; 7(7):228.
- The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells.
Wang F, Koyama N, Nishida H, Haraguchi T, Reith W, Tsukamoto T.
Molecular and Cellular Biology 2006 Jun; 26(11):4028.
Application：IF, ChIP, Mouse, Clone 2 cells.
- Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins.
Kametaka A, Takagi M, Hayakawa T, Haraguchi T, Hiraoka Y, Yoneda Y.
Genes to Cells 2002 Dec; 7(12):1231.
Application：IF, Human, HeLa cells.
- The Heterochromatin Protein 1 family.