This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice. Working dilutions in non-precipitating antibody-binding techniques: 1/10,000-1/60,000.
Reactivity
Bovine
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Catalase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity
The antibody recognizes catalase from Bovine Liver. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Buffer
PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized Hyperimmune IgG fraction
Reconstitution Method
Restore by adding 1.0 ml of sterile distilled water
Concentration
lot specific
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation
Unconjugated
Storage
Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
