WB analysis of Nocodazole-treated Cos-7 whole cell lysate using GTX85607 Aurora B (phospho Thr232) antibody prior incubated with (Lane 2) or without (Lane 1) blocking peptide.
Dilution : 1:500

IHC-P analysis of human placenta tissue (pH 9) using GTX85607 Aurora B (phospho Thr232) antibody.
Antigen retrieval : not performed.
Dilution : 10 μg/mL

IHC-P analysis of human intestine tissue (pH 9) using GTX85607 Aurora B (phospho Thr232) antibody.
Antigen retrieval : not performed.
Dilution : 10 μg/mL

Human Tonsil (formalin-fixed, paraffin-embedded) stained with AURKB antibody (GTX85607) at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.

Western Blot shows detection of Aurora B protein at 39 kD (predicted band size). All lanes: Aurora B (phospho T232) antibody diluted 1:500.
Lane 1: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs)
Lane 2: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) and with the phosphopeptide immunogen