- Catalogue No.
K098
- Size
96T(43 samples)
- Range
1.10-72.30IU/L
- Sensitivity
1.10IU/L
- Kit component
Item
Component
Size（96T）
Storage
Reagent 1
Buffer Solution
0.5 mL
2-8℃
Reagent 2
2 mmol/L Sodium Pyruvate
0.5 mL
2-8℃
Reagent 3
Substrate Solution
5 mL
2-8℃
Reagent 4
Chromogenic Agent
5 mL
2-8℃shading light
Reagent 5
Alkali Reagent
5 mL
2-8℃
Product Description
1 copy
- Storage
The kit should be stored at 2-8℃ shading light for 6 months.
- Principle of the Assay
AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitro phenyl hydrazine(DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
Cat# K098-96T(43samples)
Size : 96T(43samples)
Brand : FineTest
Contact local distributor :
Phone : +1 850 650 7790
Inquiry
Dispatch Time: About 3 working days
