Anti-Psc | Polycomb group protein Psc
Cat# AS174129
Size : 50ug
Brand : Agrisera
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Anti-Psc | Polycomb group protein Psc
Product no: AS17 4129
AS17 4129 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Drosophila melanogaster
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| Immunogen: | GST-conjugated, full length Psc protein of Drosophila melanogaster, UniProt: P35820 |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified serum in PBS pH 7.4. |
| Format: | Lyophilized |
| Quantity: | 50 µg |
| Reconstitution: | For reconstitution add 50 µl of sterile water |
| Storage: | Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Chromating Immunoprecipitation (ChIP) |
| Expected | apparent MW: | 169 | 190 kDa |
| Confirmed reactivity: | Drosophila melanogaster |
 ChIP and qPCR analysis were done as described (Schwartz et al., 2006. doi: 10.1038/ng1817). Chromatin from Psc/Su(z)2-KO cells (Kahn et al., 2016. doi: 10.1093/nar/gkw701 ) served as a negative control (Psc minus), chromatin from Ras3 cells served as a positive control (WT). Quantitative PCR was performed with primers specific for BXD-PRE of the Ubx gene (Polycomb target gene in the repressed state) and for an intergenic region (negative control). Figure 1 shows the ChIP recovery, measured by qPCR as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA). Chromatin from 5x107 cells and 3g of anti-Psc antibody were used for each ChIP reaction. For ChIP 3µg of anti-Psc antibody is recommended. Recombinant protein GST-Psc containing 822-1021 aa of Psc / UniProtKB - P35820 (PSC_DROME) / was used as immunogen. Courtesy of Dr. Tatyana Khan, Umeå University, Sweden. |
| Additional information (application): | Reactivity of this antibody in Western blot requires to be determined |
| Background: | Psc is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Alternative names: Protein posterior sex combs |