| Short Description : | Goat polyclonal antibody anti-PCNA, Biotinylated (111-122) is suitable for use in ELISA and Western Blot research applications. |
| Applications: | Pep-ELISA/WB |
| Host: | Goat |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Biotin |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1). The observed molecular weight corresponds to earlier findings in l |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | |
| Gene ID: | |
| Uniprot ID: | |
| Accession Number: | NP_002583.1 |
| Immunogen Region: | 111-122 |
| Immunogen Sequence: | EAPNQEKVSDYE. |
| Specificity: | Reported variants represent identical protein: NP_872590.1, NP_002583.1 |
| Function | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Protein Name | Proliferating Cell Nuclear AntigenPcnaCyclin |
| Database Links | |
| Cellular Localisation | NucleusColocalizes With CrebbpEp300 And Pold1 To Sites Of Dna DamageForms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S PhaseCo-Localizes With Smarca5/Snf2h And Baz1b/Wstf At Replication Foci During S PhaseTogether With Apex2Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents |
| Alternative Antibody Names | Anti-Proliferating Cell Nuclear Antigen antibodyAnti-Pcna antibodyAnti-Cyclin antibodyAnti-PCNA antibody |
Information sourced from Uniprot.org