Size : 5mg
Phone : +1 850 650 7790
Synonyms:
| Description | Anti-inflammatory agent 50 (compound a1), a derivative of Fusidic acid, exerts its effects through inhibition of inflammatory mediators including NO, IL-6, and TNF-α. It mitigates acute lung injury by modulating these mediators and suppressing the MAPK, NF-κB, and NLRP3 inflammasome signaling pathways [1]. |
| In vitro | In LPS-stimulated RAW264.7 cells, Anti-inflammatory agent 50 (compound a1) inhibits inflammatory mediators including nitric oxide (NO) with an IC50 of 3.26 μM, interleukin-6 (IL-6) with an IC50 of 1.85 μM, and tumor necrosis factor-alpha (TNF-α) with an IC50 of 3.88 μM. Additionally, Anti-inflammatory agent 50 significantly suppresses the expression of certain immunorelated cytotoxic factors, such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) [1]. |
| In vivo | Anti-inflammatory agent 50 (compound a1) mitigates pulmonary inflammation and decreases levels of NO, IL-6, TNF-α, COX-2, and iNOS in mice with LPS-induced acute lung injury (ALI). It accomplishes this by attenuating the phosphorylation of p38 MAPK, c-JNK, and ERK, thereby inhibiting the MAPK signaling pathway. Additionally, Anti-inflammatory agent 50 inhibits the NF-κB signaling pathway by preventing the phosphorylation of IκBα [1]. |
| Molecular Weight | 673.88 |
| Formula | C40H55N3O6 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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