| Short Description : | Rabbit polyclonal HK2 (1-120) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | |
| Gene ID: | |
| Uniprot ID: | |
| Immunogen Region: | 1-120 |
| Immunogen Sequence: | MIASHLLAYFFTELNHDQVQ KVDQYLYHMRLSDETLLEIS KRFRKEMEKGLGATTHPTAA VKMLPTFVRSTPDGTEHGEF LALDLGGTNFRVLWVKVTDN GLQKVEMENQIYAIPEDIMR |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2). |
| Tissue Specificity | Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. |
| Function | Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis. |
| Protein Name | Hexokinase-2Hexokinase Type IiHk IiHexokinase-BMuscle Form Hexokinase |
| Database Links | |
| Cellular Localisation | Mitochondrion Outer MembranePeripheral Membrane ProteinCytoplasmCytosolThe Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer MembraneThe Interaction With The Mitochondrial Outer Membrane Via The Mitochondrial-Binding Peptide (Mbp) Region Promotes Higher Stability Of The ProteinRelease From The Mitochondrial Outer Membrane Into The Cytosol Induces Permeability Transition Pore (Ptp) Opening And Apoptosis |
| Alternative Antibody Names | Anti-Hexokinase-2 antibodyAnti-Hexokinase Type Ii antibodyAnti-Hk Ii antibodyAnti-Hexokinase-B antibodyAnti-Muscle Form Hexokinase antibodyAnti-HK2 antibody |
Information sourced from Uniprot.org