| Short Description : | Rabbit polyclonal DDX41 (1-300) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | |
| Gene ID: | |
| Uniprot ID: | |
| Immunogen Region: | 1-300 |
| Immunogen Sequence: | MEESEPERKRARTDEVPAGG SRSEAEDEDDEDYVPYVPLR QRRQLLLQKLLQRRRKGAAE EEQQDSGSEPRGDEDDIPLG PQSNVSLLDQHQHLKEKAEA RKESAKEKQLKEEEKILESV AEGRALMSVKEMAKGITYDD PIKTSWTPPRYVLSMSEERH ERVRKKYHILVEGDGIPPPI KSFKEMKFPAAILRGLKKKG IHHPTPIQIQGIPTILSGRD MIGIAFTGSGKTLVFTLPV |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2). |
| Post Translational Modifications | Acetylation at Lys-9 regulates the nuclear/cytoplasmic localization. Phosphorylated by BTK.phosphorylation induces binding to dsDNA and STING1. 'Lys-48'-linked ubiquitinated and degraded by TRIM21 leading to negative regulation of the innate immune response to intracellular dsDNA. |
| Function | Multifunctional protein that participates in many aspects of cellular RNA metabolism. Plays pivotal roles in innate immune sensing and hematopoietic homeostasis. Recognizes foreign or self-nucleic acids generated during microbial infection, thereby initiating anti-pathogen responses. Mechanistically, phosphorylation by BTK allows binding to dsDNA leading to interaction with STING1. Modulates the homeostasis of dsDNA through its ATP-dependent DNA-unwinding activity and ATP-independent strand-annealing activity. In turn, induces STING1-mediated type I interferon and cytokine responses to DNA and DNA viruses. Selectively modulates the transcription of certain immunity-associated genes by regulating their alternative splicing. Binds to RNA (R)-loops, structures consisting of DNA/RNA hybrids and a displaced strand of DNA that occur during transcription, and prevents their accumulation, thereby maintaining genome stability. Also participates in pre-mRNA splicing, translational regulation and snoRNA processing, which is essential for ribosome biogenesis. |
| Protein Name | Probable Atp-Dependent Rna Helicase Ddx41Dead Box Protein 41Dead Box Protein Abstrakt Homolog |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmPredominantly Present In The Nucleus And Traffics To The CytoplasmSpecifically In The Perinuclear RegionAfter Dna Stimulation |
| Alternative Antibody Names | Anti-Probable Atp-Dependent Rna Helicase Ddx41 antibodyAnti-Dead Box Protein 41 antibodyAnti-Dead Box Protein Abstrakt Homolog antibodyAnti-DDX41 antibodyAnti-ABS antibody |
Information sourced from Uniprot.org