Anti-Collagen 1 alpha 1 Antibody

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Cat# CPA3566-100ul

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : Cohesion Biosciences

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Contact local distributor :

Phone : +1 850 650 7790

Western blot analysis of Collagen 1 alpha 1 expression in A549 (A), mouse kidney (B), mouse testis (C), rat kidney (D), rat testis (E) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 160 kD)
Immunohistochemical analysis of Collagen 1 alpha 1 Antibody staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Immunofluorescent analysis of Collagen 1 alpha 1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-Collagen 1 alpha 1 Antibody
Cat No:	CPA3566
Source:	Rabbit
Reactivity:	H, M, R, B, D
Applications:	WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to Collagen 1 alpha 1

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Collagen 1 alpha 1. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of Collagen 1 alpha 1 protein.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene Symbol：COL1A1

Alternative Names：Collagen alpha-1(I) chain; Alpha-1 type I collagen

Entrez Gene (Human)： 1277;

Entrez Gene (Mouse)： 12842;

Entrez Gene (Rat)： 29393;

SwissProt (Human)： P02452;

SwissProt (Mouse)： P11087;

SwissProt (Rat)： P02454;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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