Anti-CD49b Antibody-APC/Cy7 labled
Cat# CFD8809-100
Size : 100Assays
Brand : Cohesion Biosciences
Contact local distributor :
Phone : +1 850 650 7790
| Product Name: | Anti-CD49b Antibody-APC-Cyanine7 labled |
| Cat No: | CFD8809 |
| Source: | Rat |
| Reactivity: | M |
| Applications: | IF/IC, FC |
| *Application Key: | |
| H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
| *Species Reactivity Key: | |
| E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
Description：Rat monoclonal antibody APC-Cyanine7 labled to CD49bImmunogen：Mouse colon carcinoma cell line Colon26Purification：The antibody was purified by affinity chromatography.Specificity：Recognizes mouse CD49bClonality：Monoclonal (clone: DX5)Conjugation：APC-Cyanine7Form：Rat IgM kappa. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.Dilution：10 μl / assayGene Symbol：ITGA2Alternative Names：CD49B; Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; CD49b Entrez Gene (Mouse)： 16398; SwissProt (Mouse)： Q62469; Directions for Use：1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Platform：Ex/Em = 651/780 nm Storage/Stability：Shipped and store at 4°C for one year. Do not freeze.