Anti-CCDC6 Antibody

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Western blot analysis of CCDC6 expression in Jurkat (A), mouse brain (B) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 56 kD)
Immunohistochemical analysis of CCDC6 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CCDC6 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-CCDC6 Antibody
Cat No:	CQA3990
Source:	Rabbit
Reactivity:	H, M
Applications:	WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to CCDC6

Immunogen：Recombinant fusion protein of human CCDC6. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of CCDC6 protein

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene Symbol：CCDC6

Alternative Names：D10S170; TST1; Coiled-coil domain-containing protein 6; Papillary thyroid carcinoma-encoded protein; Protein H4

Entrez Gene (Human)： 8030;

Entrez Gene (Mouse)： 76551;

SwissProt (Human)： Q16204;

SwissProt (Mouse)： D3YZP9;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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