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> Anti-Beta-tubulin Antibody

Anti-Beta-tubulin Antibody

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Cat# CPA9126-100ul

Size : 100ul

Brand : Cohesion Biosciences

Contact local distributor :


Phone : +1 850 650 7790

  • Western blot analysis of Beta-tubulin expression in A549 (A), mouse brain (B), rat brain (C), chicken lung (D), rabbit testis (E), sheep muscle (F) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 52 kD)
  • Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of Beta-tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-Beta-tubulin Antibody
Cat No:CPA9126
Source:Mouse
Reactivity:H, M, R, Mk, D, C, Rb, S, Insect, Yeast
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Mouse monoclonal antibody to Beta-tubulin
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary.
Purification：Affinity chromatography
Specificity：Recognizes endogenous levels of Beta-tubulin protein.
Clonality：Monoclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/2000 - 1/5000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
Gene Symbol：TUBB3
Alternative Names：TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Entrez Gene (Human)： 10381;
Entrez Gene (Mouse)： 22152;
Entrez Gene (Rat)： 246118;
SwissProt (Human)： Q13509;
SwissProt (Mouse)： Q9ERD7;
SwissProt (Rat)： Q4QRB4;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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CPA9126-200ul
Anti-Beta-tubulin Antibody
 200ul