Anti-Acetylated-Histone H3 Antibody (Lys 4)
Cat# OAAI00724
Size : 100ug
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Anti-Acetylated-Histone H3 Antibody (Lys 4) (OAAI00724)
| Datasheets/Manuals | Printable datasheet for Anti-Acetylated-Histone H3 Antibody (Lys 4) (OAAI00724) |
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| Predicted Species Reactivity | Human |
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| Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Application | ELISA, FC, IHC-Fr, IP |
| Additional Information | Background: Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present. |
| :: | Positive Control: 3T3 Nuclear Extract |
| :: | Cellular Location: Nucleus |
| Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
| Immunogen | A synthetic peptide containing ART[Acetyl-K]QTAR in which Acetylation site is located at lysine 4 of human histone H3. |
| Purification | Affinity Purified |
| Concentration | 100ug/200ul |
| Target Post-Translational Modification | Acetylation |
| Specificity | H3C1 |
| Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
| Reference | Vakoc C et al. Histone H3 Lysine 9 Methylation and HP1γ Are Associated with Transcription Elongation throμgh Mammalian Chromatin . Molecular Cell (2003), Volume 19 , Issue 3 , Pages 381 - 391. David J Seward, et al. Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins. Nature Structural & Molecular Biology 14, 240 - 242 (2007) |
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| Gene Symbol | H3C1 |
| Gene Full Name | H3 clustered histone 1 |
| Alias Symbols | H3 histone family, member A;H3/A;H3C10;H3C11;H3C12;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;HIST1H3A;histone 1, H3a;histone cluster 1 H3 family member a;histone cluster 1, H3a;histone H3.1;histone H3/a. |
| NCBI Gene Id | 8350 |
| Description of Target | Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This Antibody is specific for histone H3 tri-methylated at K4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The Antibody will react with any of the above species where the trimethylation modification is present. |
| Uniprot ID | P68431 |
| Molecular Weight | 17 |
