Reagents and instruments for immunology, cell biology and molecular biology.
 
> Ac-DEVD-AFC [201608-14-2]

Ac-DEVD-AFC [201608-14-2]

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Cat# HY-P1005-1mg

Size : 1mg

Brand : MedChemExpress

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Phone : +1 850 650 7790

Description

Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).

In Vitro

After incubation with Ac-DEVD-AFC for 1 hour, significant increase of caspase-3 activity is observed at 4 hour compare with control. There are no significant increases of caspase-3 activity in Photofrin and LPLI group. The cleavage of Ac-DEVD-AFC in response to caspase-3 activation is remarkably inhibited by shRNA-BimL transfection[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ac-DEVD-AFC Related Antibodies

Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (68.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3706 mL 6.8530 mL 13.7060 mL
5 mM 0.2741 mL 1.3706 mL 2.7412 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

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