5X Passive Lysis Buffer

Cat# 99912

Size : 30ML

Brand : Biotium

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5X Passive Lysis Buffer
Storage Conditions

Store at -10 to -35 °C or below

Product Description

5X Passive Lysis Buffer is concentrated lysis buffer designed for use with our Renilla Luciferase Assays when additional volumes of lysis buffer are required.

  • Additional 5X Passive Lysis Buffer for larger volume assays
  • For use with Renilla Luciferase Assay Kit 2.0 (30082)

Our kits are supplied with sufficient 5X Passive Lysis Buffer to complete the stated number of assays using 96-well to 24-well plates. 5X Passive Lysis Buffer is sold separately for use with the kits when more lysis buffer (e.g., >100 uL per well) is required. If you require additional lysis buffer for use with Firefly & Renilla Luciferase Single Tube Assay Kit (30081),  use 1X Passive Lysis Buffer 2.0 (99821-100mL). If you require additional lysis buffer for use with Firefly Luciferase Assay Kit 2.0 (30085) or Firefly Luciferase Assay Kit (Lyophilized) (30075), use 5X Firefly Luciferase Lysis Buffer (99923).

More Luciferase Kits

Biotium Cat. No. Product Description Promega Assay Promega Cat. No.
30138 Steady-ATP™ HTS Viability Assay Kit Highly sensitive ATP-based cell luminescent viability assay with signal half-life of 5 hours for high-throughput screening in multi-well plates. CellTiter-Glo® Luminescent Cell Viability Assay G7570, G7571,
G7572, G7573
30085 Firefly Luciferase Assay Kit 2.0 Flash-type firefly luciferase assay kit. Firefly Luciferase Assay System E1483, E1500,
E1501, E4030,
E4530, E4550
30075 Firefly Luciferase Assay Kit (Lyophilized) Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
30028 Steady-Luc™ Firefly HTS Assay Kit Glow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates. Steady-Glo® Assay System E2510, E2520, E2550
30028L Steady-Luc Firefly HTS Assay Kit (Lyophilized) Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
30082 Renilla Luciferase Assay Kit 2.0 Flash-type Renilla luciferase assay kit. Renilla Luciferase Assay System E2810, E2820
30081 Firefly and Renilla Luciferase Single Tube Assay Kit Assay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube. Dual-Luciferase® Assay System
(Firefly & Renilla)
E1910, E1960, E1980

References

  1. Biochem J, 477, 137 (2020), DOI: 10.1042/BCJ20190596
  2. PLoS ONE, 14(2), e0212850 (2019), DOI: 10.1371/journal.pone.0212850
  3. J Tradit Complement Med, 9, 227, (2018), DOI: 10.1016/j.jtcme.2017.07.004
  4. Int. J. Mol. Sci, 19, 774, (2018), DOI: 10.3390/ijms19030774
  5. Int Heart J, 59, 190, (2018), DOI: 10.1536/ihj.16-372
  6. Front. Immunol., 9,1564, (2018), DOI: 10.3389/fimmu.2018.01564
  7. J. Orthop. Res., 35, 876, (2017), DOI: 10.1002/jor.23342
  8. Bulletin Chem Soc. J (2016), DOI: 10.1246/bcsj.20150405
  9. PLoS ONE, 8(1), e53936 (2013), DOI: 10.1371/journal.pone.0053936
  10. Biol Syst 1, 1, (2012), DOI: 10.4172/bso.1000104
  11. Int J Biochem Mol Biol, 2(4), 318, (2011), PMCID: PMC3242428
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