Reagents and instruments for immunology, cell biology and molecular biology.
 
ABC detection kits - Rabbit - Horseradish Peroxidase (HRP)

ABC detection kits - Rabbit - Horseradish Peroxidase (HRP)

ABC detection kits for rabbit antibodies employ the avidin-biotin binding mechanism to amplify immunodetection signals. The system starts with a primary antibody raised in rabbit binding to a specific antigen. A biotinylated secondary antibody specific for rabbit IgG then binds the primary antibody. The avidin-biotin complex is formed by binding avidin conjugated to horseradish peroxidase (HRP) enzyme to the biotin on the secondary antibody, resulting in signal amplification at the antigen site.

Enzymatic Signal Generation

Horseradish peroxidase catalyzes the oxidation of chromogenic substrates such as diaminobenzidine (DAB), resulting in a brown, insoluble precipitate that deposits precisely where antigen-antibody complexes are located. This allows visualization under bright-field microscopy for immunohistochemistry (IHC), immunocytochemistry (ICC), ELISA, and blotting applications.

Applications and Versatility

This detection method is extensively used in biomedical research and clinical diagnostics to detect antigens with rabbit primary antibodies. It supports formalin-fixed paraffin-embedded tissues, frozen samples, and various cellular preparations. The system's high sensitivity and specificity make it ideal for detecting proteins with low abundance or in samples with high endogenous peroxidase activity where other enzymatic detections may fail.

Advantages of Rabbit ABC-HRP Kits

  • High Sensitivity: Multiple HRP enzyme molecules bound to avidin increase the enzymatic activity and enhance signal intensity, allowing detection of low-level antigens.

  • Fast Color Development: HRP provides rapid substrate conversion enabling quicker visualization than alkaline phosphatase-based systems.

  • Stable and Reproducible: The avidin-biotin interaction ensures stable complex formation and consistent detection results.

  • Low Background: Optimized blocking reagents help reduce nonspecific staining despite the presence of endogenous biotin or peroxidases in tissue.

Protocol Overview

A typical staining protocol involves blocking endogenous peroxidase activity, incubation with rabbit primary antibodies, binding with biotinylated anti-rabbit secondary antibodies, ABC-HRP complex addition, and chromogen substrate development. The process can be adjusted for intensity and specificity by varying incubation times and substrate choices.

Frequently Asked Questions (FAQ)

How can I quickly find a horseradish peroxidase (hrp) product?

To find a horseradish peroxidase (hrp) product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Quimigen stock several product codes in the horseradish peroxidase (hrp) category?

Yes, Quimigen distributes several product references in the horseradish peroxidase (hrp) category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for horseradish peroxidase (hrp) products?

Yes, you can request a quote for horseradish peroxidase (hrp) products from the product page or by contacting Quimigen (info@quimigen.pt). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a horseradish peroxidase (hrp) product is available to order?

The availability of a horseradish peroxidase (hrp) product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Quimigen with the product code or name of the product you are looking for.

What should I do if I cannot find the horseradish peroxidase (hrp) product I am looking for?

If the horseradish peroxidase (hrp) product you are looking for does not appear in the catalogue, please contact Quimigen with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all horseradish peroxidase (hrp) products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.

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