F(ab')2 fragment secondary antibodies

Secondary antibodies can be classified based on their specificity, which can be broad or narrow, and their fragment type, which can be whole, F(ab), or F(ab')2.

The principle of F(ab')2 is to generate divalent antibody fragments that retain the antigen-binding Fab portions of whole IgG antibodies while removing most of the Fc region.

F(ab')2 fragments are generated by pepsin digestion of whole IgG antibodies to cleave the hinge region and leave intact some of the Fc region. The resulting fragments have two antigen-binding Fab portions linked together by disulfide bonds, making them divalent with a molecular weight of about 110 kDa. The use of F(ab')2 fragments has several advantages over whole IgG antibodies, including better penetration into tissues, reduced nonspecific binding to Fc receptors on live cells or to Protein A/G, and the ability to precipitate antigens. However, F(ab')2 fragments are not recommended for blocking since they have two binding sites that are available to capture the primary antibody introduced subsequently. The preparation of F(ab')2 fragments involves pepsin digestion of whole IgG antibodies, followed by purification using techniques such as FPLC with DEAE-

Frequently Asked Questions (FAQ)

How can I quickly find a f(ab')2 product?

To find a f(ab')2 product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Quimigen stock several product codes in the f(ab')2 category?

Yes, Quimigen distributes several product references in the f(ab')2 category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for f(ab')2 products?

Yes, you can request a quote for f(ab')2 products from the product page or by contacting Quimigen (info@quimigen.pt). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a f(ab')2 product is available to order?

The availability of a f(ab')2 product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Quimigen with the product code or name of the product you are looking for.

What should I do if I cannot find the f(ab')2 product I am looking for?

If the f(ab')2 product you are looking for does not appear in the catalogue, please contact Quimigen with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all f(ab')2 products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.