Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using WNK1 polyclonal antibody (Cat # PAB18141).
Peptide "+" means "peptide blocking".
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells treated with EGF (200 ng/mL, 30 mins), using WNK1 polyclonal antibody (Cat # PAB18141).
Peptide "+" means "peptide blocking".
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of WNK1.
Immunogen
A synthetic peptide corresponding to human WNK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to WNK1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:300-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using WNK1 polyclonal antibody (Cat # PAB18141).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells treated with EGF (200 ng/mL, 30 mins), using WNK1 polyclonal antibody (Cat # PAB18141).
Peptide "+" means "peptide blocking". - Gene Info — WNK1
Entrez GeneID
65125Protein Accession#
Q9H4A3Gene Name
WNK1
Gene Alias
KDP, KIAA0344, PHA2C, PRKWNK1, PSK
Gene Description
WNK lysine deficient protein kinase 1
Gene Ontology
HyperlinkGene Summary
The WNK1 gene encodes a cytoplasmic serine-threonine kinase expressed in distal nephron.[supplied by OMIM
Other Designations
OTTHUMP00000115370|kinase deficient protein|prostate-derived sterile 20-like kinase|protein kinase, lysine deficient 1|serine/threonine-protein kinase WNK1
- Interactomes
- Diseases
- Publication Reference
- Molecular transport machinery involved in orchestrating luminal acid-induced duodenal bicarbonate secretion in vivo.
Singh AK, Liu Y, Riederer B, Engelhardt R, Thakur BK, Soleimani M, Seidler U.
The Journal of Physiology 2013 Nov; 591(21):5377.
Application：IF, Mouse, Apical enterocyte cells.
- Acute growth hormone administration induces antidiuretic and antinatriuretic effects and increases phosphorylation of NKCC2.
Dimke H, Flyvbjerg A, Bourgeois S, Thomsen K, Frokiaer J, Houillier P, Nielsen S, Frische S.
American Journal of Physiology. Renal Physiology 2007 Feb; 292(2):F723.
- The Na+:Cl- cotransporter is activated and phosphorylated at the amino-terminal domain upon intracellular chloride depletion.
Pacheco-Alvarez D, Cristobal PS, Meade P, Moreno E, Vazquez N, Munoz E, Diaz A, Juarez ME, Gimenez I, Gamba G.
The Journal of Biological Chemistry 2006 Sep; 281(39):28755.
- Molecular transport machinery involved in orchestrating luminal acid-induced duodenal bicarbonate secretion in vivo.