Purified Vicia villosa lectin (VVA), Biotin conjugated.

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Carbohydrate Specificity:
N-Acetylgalactosamine

Inhibitory Carbohydrate:
N-Acetylgalactosamine

Activity:
More than 250ug/ml is usually required to agglutinate type A1 cells. Less than 1ug/ml will agglutinate neuraminidase treated cells regardless of blood type.

Chemical Used for Conjugation:
Biotinyl N-hydroxysuccinimide ester (BNOHSE)

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.