Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques). Recommended working dilutions: vary widely, but may be between 1/10000 and 1/50000.
Reactivity
Bakers Yeast
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Triosephosphate isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Buffer
PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized IgG fraction Label: Marker: N-Hydroxysuccinimidobiotin. Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilised to assure stability and long shelf life Molar radio: Biotin/ IgG ~5.9
Reconstitution Method
Restore by adding 1.0 ml of sterile distilled water
Concentration
lot specific
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation
Biotin
Storage
Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
