Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human placenta stained with TGFB2 polyclonal antibody (Cat # PAB12739) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TGFB2.
Immunogen
A synthetic peptide corresponding to C-terminus of human TGFB2.
Host
Rabbit
Theoretical MW (kDa)
~50
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~50 kDa of TGFB2, not TGFB1 and less extense of TGFB3.
Form
Liquid
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Immunohistochemistry (2-5 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (antibody stabilizer).
Storage Instruction
Store at 4°C for one month. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months. - Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human placenta stained with TGFB2 polyclonal antibody (Cat # PAB12739) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Enzyme-linked Immunoabsorbent Assay
Immunoprecipitation
Western Blot
- Gene Info — TGFB2
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Delivery of LOXL1-AS1-siRNAs using targeting peptide-engineered extracellular vesicles with focused ultrasound to suppress medulloblastoma metastasis.
Anh Duy Do, Shing-Shung Chu, Le Hien Giang, Pei-Lin Huang, Yu-Ling Lin, Che-Chang Chang, Tai-Tong Wong, Shian-Ying Sung, Chia-Ling Hsieh.
Journal of Nanobiotechnology 2025 Jun; 23(1):460.
Application：IHC, Mice, NOD/SCID mice.
- Divergent expression of liver transforming growth factor superfamily cytokines after successful portoenterostomy in biliary atresia.
Kerola A, Lohi J, Heikkilä P, Mutanen A, Jalanko H, Pakarinen MP.
Surgery 2019 May; 165(5):905.
Application：IHC-P, Human, Human livers.
- Delivery of LOXL1-AS1-siRNAs using targeting peptide-engineered extracellular vesicles with focused ultrasound to suppress medulloblastoma metastasis.