TET1 Antibody
Cat# OAGA08457
Size : 100ul
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for TET1 Antibody (OAGA08457) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid |
| Clonality | Monoclonal |
| Clone | GT465 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | Unconjugated |
| Application | ICC|IF|WB |
| Reconstitution and Storage | -20°C or -80°C|2°C to 8°C |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary. |
| Purification | Protein G Purified |
| Concentration | 1 mg/ml |
| Application Info | Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Storage Buffer | PBS |
| Gene Symbol | TET1 |
|---|---|
| Gene Full Name | tet methylcytosine dioxygenase 1 |
| Alias Symbols | bA119F7.1, CXXC finger 6, CXXC zinc finger 6, CXXC-type zinc finger protein 6, CXXC6, LCX, leukemia-associated protein with a CXXC domain, methylcytosine dioxygenase TET1, ten-eleven translocation 1 gene protein, ten-eleven translocation-1, tet oncogene 1, TET1 splice variant VP_DE4, TET1 splice variant VP_DE456. |
| NCBI Gene Id | 80312 |
| Protein Name | Methylcytosine dioxygenase TET1 |
| Description of Target | Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an important role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789). |
| Uniprot ID | Q8NFU7 |
| Protein Accession # | NP_085128.2 |
| Nucleotide Accession # | NM_030625.2 |
