Flow Cytometry
BALB/c splenocytes were double-stained with Tcrb monoclonal antibody, clone H57-597 (FITC) (Cat # MAB5763) and Cd19 monoclonal antibody, clone 6D5 (PE) (Cat # MAB5689), gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
- Specifications
Product Description
Hamster monoclonal antibody raised against native Tcrb.
Immunogen
Native purified mouse Tcrb.
Host
Hamster
Reactivity
Mouse
Specificity
Common epitope of the beta chain of the mouse alphabeta T cell receptor (TCR) complex.
Form
Liquid
Conjugation
PE/Cy5.5
Isotype
IgG
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Flow Cytometry
BALB/c splenocytes were double-stained with Tcrb monoclonal antibody, clone H57-597 (FITC) (Cat # MAB5763) and Cd19 monoclonal antibody, clone 6D5 (PE) (Cat # MAB5689), gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). - Gene Info — Tcrb
- Publication Reference
- Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes.
S Murosaki, Y Yoshikai, A Ishida, T Nakamura, G Matsuzaki, H Takimoto, H Yuuki, K Nomoto.
International Immunology 1991 Oct; 3(10):1005.
Application：Flow Cyt, Mouse, Mouse intestinal intra-epithelial lymphocytes.
- In vivo administration of monoclonal antibodies to the CD3 T cell receptor complex induces cell death (apoptosis) in immature thymocytes.
Shi YF, Bissonnette RP, Parfrey N, Szalay M, Kubo RT, Green DR.
Journal of Immunology 1991 May; 146(10):3340.
- Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.
Gascoigne NR.
The Journal of Biological Chemistry 1990 Jun; 265(16):9296.
- Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes.