TCPTP (PTPN2) (NM_080422) Human Tagged ORF Clone
Cat# RC202161
Size : 10ug
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TCPTP (PTPN2) (NM_080422) Human Tagged ORF Clone
- TrueORF®
SKU
RC202161
PTPN2 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 2 (PTPN2), transcript variant 2
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TCPTP |
| Synonyms | PTN2; PTPT; TC-PTP; TCELLPTP; TCPTP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | Plasmid Map  |
| ACCN | NM_080422 |
| ORF Size | 1161 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@quimigen.pt or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_080422.2, NP_536347.1 |
| RefSeq Size | 1714 bp |
| RefSeq ORF | 1164 bp |
| Locus ID | 5771 |
| UniProt ID | P17706 |
| Cytogenetics | 18p11.21 |
| Domains | PTPc_motif, Y_phosphatase |
| Protein Families | Druggable Genome, Phosphatase, Transmembrane |
| MW | 45 kDa |
| Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. provided by RefSeq, May 2011 |
| Product Manuals |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC202161L3 | Lenti-ORF clone of PTPN2 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 2 (PTPN2), transcript variant 2 | 10 ug | | |
| RC202161L4 | Lenti-ORF clone of PTPN2 (mGFP-tagged)-Human protein tyrosine phosphatase, non-receptor type 2 (PTPN2), transcript variant 2 | 10 ug | | |
| RG202161 | PTPN2 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 2 (PTPN2), transcript variant 2 | 10 ug | | |
| SC310456 | PTPN2 (untagged)-Human protein tyrosine phosphatase, non-receptor type 2 (PTPN2), transcript variant 2 | 10 ug | | |
Citations
Loss of Function Mutation in PTPN2 Causes Aberrant Activation of JAK Signaling via STAT and Very Early Onset Intestinal Inflammation
Parlato, Nian, Charbit-Henrion et al
Gastroenterology (2020)
Parlato, Nian, Charbit-Henrion et al
Gastroenterology (2020)
Cited by 44
AI Summary: OriGene products, including the FLAG-WT-PTPN2 and FLAG-C216G-PTPN2 expression vectors as well as an anti-FLAG mouse monoclonal antibody, were used in this study to express and immunoprecipitate PTPN2 variants for functional analysis in Lenti-X™293T cells.
Snippet: Lenti-X 293T cells were transfected with FLAG-WT-PTPN2, FLAG-C216G-PTPN2 or empty vector (EV) (Origene). 24h post-transfection, cells were washed with PBS and resuspended in lysis buffer (PBS, 0.5% Triton X-100, protease inhibitors cocktail). … For PTPN2 immunoprecipitation, 1 mg of whole-cell extracts (200 μl) were incubated overnight with 1 μg of an anti-flag mouse monoclonal antibody (Origene) at 4 °C. PTPN2 catalytic activity assay toward FAM-pStat1 peptide in immunoprecipitate was performed as previously described5 . For testing dominant negative activity, untagged recombinant WT and C216G PTPN2 were produced in E. coli as described56 and mixed at different ratios to reach a total concentration of 50 nM in 100 mM sodium acetate, pH6 buffer containing 10 mM p-nitrophenyl phosphate. …
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