RM RANK LIGAND
Cat# 200-04-1MG
Size : 1MG
Brand : FUJIFILM
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Phone : +1 850 650 7790
Recombinant Mouse RANK Ligand
Receptor activator of nuclear factor kappa-B Ligand (RANK Ligand) is a cell-bound marker related to the tumor necrosis factor (TNF) family of proteins. RANK Ligand plays a critical role in bone metabolism and osteoclast differentiation. T cell expression of RANK Ligand promotes dendritic cell maturation.
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Single Prominent Band at 21.0 kDa
Figure 1. rm Rank-Ligand (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 21.0 kDa
RANKL stimulation using modified cell line
Figure 2. Recombinant mouse RANKL activity induced bioluminiscence upon binding to receptor on RANKL BioAssay cells
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | TNFSF11, TRANCE, OPGL, ODF |
| Accession Number | O35235 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 19.5 kDa (175 aa) |
| AA Sequence | MPAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 20 mM sodium chloride, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 1 EU/µg |
| Biological Activity (ED50) | Ability to induce bioluminescence in engineered RANKL bioassay cells | ≤ 50 ng/mL |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.