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> Reston ebolavirus (REBOV) (strain Philippines-96) Nucleoprotein/NP Protein (His & SUMO)

Reston ebolavirus (REBOV) (strain Philippines-96) Nucleoprotein/NP Protein (His & SUMO)

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Cat# NB-64-52074-20ug

Size : 20ug

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Reston ebolavirus (REBOV) (strain Philippines-96) Nucleoprotein/NP Protein (His & SUMO)

(Synonyms: Reston NP (rNP), Nucleoprotein, Nucleocapsid protein (Protein N), NP) Copy Product Info

Synonyms: Reston NP (rNP), Nucleoprotein, Nucleocapsid protein (Protein N), NP

Catalog No. TMPH-03405 Copy Product Info
Reston ebolavirus (REBOV) (strain Philippines-96) Nucleoprotein/NP Protein (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 53.3 kDa and the accession number is Q91DE1.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Reston ebolavirus (REBOV) (strain Philippines-96) Nucleoprotein/NP Protein (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 53.3 kDa and the accession number is Q91DE1.
Species
REBOV
Expression System
E. coli
TagN-6xHis-SUMO
Accession NumberQ91DE1
Amino AcidRPNLGSRQDDDNEIPFPGPISNNPDQDHLEDDPRDSRDTIIPNSAIDPEDGDFENYNGYHDDEVGTAGDLVLFDLDDHEDDNKAFELQDSSPQSQREIERERLIHPPPGNNKDDNRASDNNQQSADSEEQEGQYNRHRGPERTTANRRLSPVHEEDTPIDQGDDDPSSPPPLESDDDDASSSQQDPDYTAVAPPAPVYRSAEAHEPPHKSSNEPAETSQLNEDPDIGQSKSMQKLGETYHHLLRTQGPFEAINYYHMMKDEPVIFSTDDGKEYTYPDSLEEAYPPWLTEKERLDNENRYIYINNQQFFWPVMSPRDKFLAILQHHQ
Construction414-739 aa
Protein Purity
> 90% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationTris-based buffer, 50% glycerol
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsReston NP (rNP), Nucleoprotein, Nucleocapsid protein (Protein N), NP
Research Background
Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm. Each NP binds to six nucleotides of viral genomic RNA, three being exposed to the solvant and three hidden into the nucleocapsid. Recruits also host PPP2R5C phosphatase to dephosphorylate VP30 and thereby promote viral transcription. Upon virion assembly and budding, NP binds to VP24 and possibly host STAU1.
Chemical Properties
Molecular Weight53.3 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Protein N
Related Tags: Reston ebolavirus (REBOV) (strain Philippines-96) Nucleoprotein/NP Protein (His & SUMO) chemical structure | Reston ebolavirus (REBOV) (strain Philippines-96) Nucleoprotein/NP Protein (His & SUMO) molecular weight