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> Recombinant Anti-p21 Rabbit mAb

Recombinant Anti-p21 Rabbit mAb

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Cat# CMA4036-50ul

Size : 50ul

Brand : Cohesion Biosciences

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Phone : +1 850 650 7790

  • Western blot analysis of p21 expression in THP1 (A) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 21 kD)
  • Immunohistochemical analysis of p21 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of p21 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Product Name:Recombinant Anti-p21 Rabbit mAb
Cat No:CMA4036
Source:Rabbit
Reactivity:H
Applications:WB, IH, IF/IC, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Recombinant rabbit monoclonal antibody to p21
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human p21. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of p21 protein
Clonality：Monoclonal
Form：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)
Gene Symbol：CDKN1A
Alternative Names：CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
Entrez Gene (Human)： 1026;
SwissProt (Human)： P38936;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.