Recombinant Anti-Aconitase 2 Rabbit mAb

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Western blot analysis of Aconitase 2 expression in MCF7 (A), K562 (B), Hela (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 85 kD)
Immunohistochemical analysis of Aconitase 2 staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Immunofluorescent analysis of Aconitase 2 staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Aconitase 2 expression in wild type (WT) and knockdown (KD) HEK293T cell lysates.

Product Name:	Recombinant Anti-Aconitase 2 Rabbit mAb [KO/KD Validated]
Cat No:	CMA4665
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IH, IF/IC, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Recombinant rabbit monoclonal antibody to Aconitase 2

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human Aconitase 2 protein. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of Aconitase 2 protein

Clonality：Monoclonal

Form：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)

Gene Symbol：ACO2

Alternative Names：Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase

Entrez Gene (Human)： 50;

Entrez Gene (Mouse)： 11429;

Entrez Gene (Rat)： 79250;

SwissProt (Human)： Q99798;

SwissProt (Mouse)： Q99KI0;

SwissProt (Rat)： Q9ER34;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.