RayBio® Rat IL-10 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Rat IL-10 ELISA Kit
Rat IL-10 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Rat |
| Sensitivity | 10 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 10 pg/ml - 6000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 - 10 fold |
| Accession Number | P29456 |
| Gene Id | 25325 |
| Gene Symbols | IL10, IL-10 |
| Protein Name / Synonyms | Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
Related Products
NexaTag® Rat IL-10 qIPCR ELISA Kit
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat IL-10 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-10 (IL10), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Preclinical evaluation of waste-derived pomegranate extract (PWE) as a potential preventing and therapeutic agent for benign prostatic hyperplasia
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (prostate tissues homogenates)
- Year:
- 2026
- Journal Name:
- Frontiers in Molecular Biosciences
- PubMed ID:
- 41797990
Melatonin promotes gut anti-oxidative status in perinatal rat by remodeling the gut microbiome
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Redox biology
CROCIN'S PROTECTIVE EFFECT AGAINST A VALPROATE-EXPOSED MODEL OF AUTISM SPECTRUM DISORDER: A HISTOLOGICAL AND OXIDO-INFLAMMATORY INVESTIGATION
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (brain)
- Year:
- 2025
- Journal Name:
- Journal of Applied Pharmaceutical Sciences and Research
Through Its Powerful Antioxidative Properties, L-Theanine Ameliorates Vincristine-Induced Neuropathy in Rats
- Species:
- Rat
- Sample Type:
- nerve tissue
- Year:
- 2023
- Journal Name:
- Antioxidants
Qinzhizhudan formula dampens inflammation in microglia polarization of vascular dementia rats by blocking MyD88/NF-κB signaling pathway: Through integrating net-work pharmacology and experimental validation
- Species:
- Rat
- Sample Type:
- brain
- Year:
- 2023
- Journal Name:
- Journal of Ethnopharmacology
Shuyu decoction exhibits anti-fatigue properties via alleviating exercise-induced immune dysfunction
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of Ethnopharmacology
Air pollution combined with high-fat feeding aggravates metabolic and cardiovascular diseases: A dangerous, oxidative, and immune-inflammatory association
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- LIFE SCIENCES
Chebulagic Acid Alleviates Inflammation Via Regulation of Skeletal Muscle IR/IRS-1/AKT/GLUT4 Signaling Pathway in Diabetic Rats
- Species:
- Rat
- Sample Type:
- Plasma
- Year:
- 2024
- Journal Name:
- Research Square
An integrated strategy to explore the potential role of melatonin against copper-induced adrenaline toxicity in rat cardiomyocytes: Insights into oxidative stress, inflammation, and apoptosis
- Species:
- Rat
- Sample Type:
- cardiomyocytes
- Year:
- 2023
- Journal Name:
- International Immunopharmacology
Human umbilical cord-derived mesenchymal stem cells improve the function of liver in rats with acute-on-chronic liver failure via downregulating Notch and Stat1/Stat3 signaling
- Species:
- Rat
- Sample Type:
- Serum (acute-on-chronic liver failure rats)
- Year:
- 2021
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 34256837
