Rat IgM Antibody : CY5
Cat# OASB00795
Size : 0.1mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Rat IgM Antibody : CY5 (OASB00795)
| Datasheets/Manuals | Printable datasheet for Rat IgM Antibody : CY5 (OASB00795) |
|---|
| Tested Species Reactivity | Rat |
|---|---|
| Predicted Species Reactivity | Rat |
| Clonality | Monoclonal |
| Clone | M2A1 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | Cyanine 5 |
| Application | FC, FLISA, IHC-F, ICC |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rat IgM hybridoma |
| Concentration | 0.1 mg/mL |
| Specificity | IgM |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested as a second step reagent byflow cytometry and/or ELISA to conform with characteristics of a standard reference reagent. Representativedata are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water.If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions.Dilute azide- containing compounds in running water before discarding to avoid accumulation of potentially explosivedeposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: ELISA: Fluorescein and Cy5 conjugates Biotin conjugate R-phycoerythrin conjugate Horseradish Peroxidase conjugate <= 0.3ug/106 cells <= 0.3 ug/106 cells <= 0.02 ug/106 cells 1:2,000-1:5,000 |
| Application Info | Identification and enumeration of IgM+ cells by flow cytometry, Identification and enumeration of IgM+ cells by immunofluorescence microscopy, Second step reagent for rat IgM monoclonal antibodies, ELISA, Surface IgM detection, B cell stimulation |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg or 0.1mg in 1.0 mL of PBS/NaN3 Store at 2- 8 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Storeat 2- 8 C. Do not freeze! - Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
