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> Rabbit Anti-MKI67 Polyclonal Antibody

Rabbit Anti-MKI67 Polyclonal Antibody

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Cat# HPA000451

Size : 100ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA000451
marker of proliferation Ki-67

Anti-MKI67 Antibody

Polyclonal Antibody against HUMAN MKI67

0.3 mg/ml
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
Anti-MKI67 Antibody
info@quimigen.pt

Immunohistochemistry analysis in human bone marrow and skeletal muscle tissues using HPA000451 antibody. Corresponding MKI67 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: marker of proliferation Ki-67
  • Target gene: MKI67
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000031004 (66%)RAT - ENSRNOG00000028137 (67%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000017932
  • Concentration: 0.3 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  

Target information

  • Protein name: marker of proliferation Ki-67
  • Gene name: MKI67
  • Alternative gene names: MIB-, PPP1R105, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (7)

  • Etzold C, Lyros O, Mehdorn M, Nowotny R, Niebisch S, Jansen-Winkeln B, Schierle K, Gockel I, Thieme RPleura Peritoneum , 2025 Feb 26; 10(1):1-9. Epub 2025 Feb 262025 Feb 26
  • Geread RS, Sivanandarajah A, Brouwer ER, Wood GA, Androutsos D, Faragalla H, Khademi ACancers (Basel) , 2020 Dec 22; 13(1):11. Epub 2020 Dec 222020 Dec 22

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