Rabbit Anti-ANXA1 Polyclonal Antibody

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Cat# HPA011272

Size : 100ul

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Phone : +1 850 650 7790

Brand : Atlas Antibodies

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Contact local distributor :

Phone : +1 850 650 7790

HPA011272

annexin A1

Anti-ANXA1 Antibody

Polyclonal Antibody against HUMAN ANXA1

0.1 mg/ml

info@quimigen.pt

Immunohistochemistry analysis in human esophagus and cerebral cortex tissues using HPA011272 antibody. Corresponding ANXA1 RNA-seq data are presented for the same tissues.

Product details

Target protein: annexin A1
Target gene: ANXA1
Verified species reactivity: Human, Mouse, Rat
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000024659 (89%)RAT - ENSRNOG00000017469 (92%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: C118952
Concentration: 0.1 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:500 - 1:1000
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody
Western Blot (WB)Genetic validation in WB by siRNA knockdown. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: annexin A1
Gene name: ANXA1
Alternative gene names: ANX1, LPC1,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (5)

Patil SS, Panchal V, Røstbø T, Romanyuk S, Hollås H, Brenk R, Grindheim AK, Vedeler AFront Cell Dev Biol , 2023 Jun 15; 11:1161588. Epub 2023 Jun 152023 Jun 15
Elakad O, Li Y, Gieser N, Yao S, Küffer S, Hinterthaner M, Danner BC, von Hammerstein-Equord A, Ströbel P, Bohnenberger HDis Markers , 2021 Apr 17; 2021:5520832. Epub 2021 Apr 172021 Apr 17