PRPF31 (NM_015629) Human Tagged ORF Clone
Cat# RC218307
Size : 10ug
Contact local distributor :
Phone :
PRPF31 (NM_015629) Human Tagged ORF Clone
SKU
RC218307
PRPF31 (Myc-DDK-tagged)-Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31)
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PRPF31 |
| Synonyms | NY-BR-99; PRP31; RP11; SNRNP61 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms | Chromatograms  Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | Plasmid Map  |
| ACCN | NM_015629 |
| ORF Size | 1497 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_015629.4 |
| RefSeq Size | 2153 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 26121 |
| UniProt ID | Q8WWY3 |
| Cytogenetics | 19q13.42 |
| Domains | Nop |
| Protein Families | Druggable Genome |
| Protein Pathways | Spliceosome |
| MW | 55.3 kDa |
| Summary | This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.provided by RefSeq, Jan 2009 |
| Product Manuals |
| FAQs |
|
| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC218307L3 | Lenti ORF clone of Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31), Myc-DDK-tagged | 10 ug | | |
| RC218307L4 | Lenti ORF clone of Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31), mGFP tagged | 10 ug | | |
| RG218307 | PRPF31 (tGFP-tagged) - Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31) | 10 ug | | |
| SC114632 | PRPF31 (untagged)-Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31) | 10 ug | | |
Citations
A CRISPR and high-content imaging assay compliant with ACMG/AMP guidelines for clinical variant interpretation in ciliopathies
Nazlamova, Thomas, Cheung et al
Hum Genet (2020)
Nazlamova, Thomas, Cheung et al
Hum Genet (2020)
Cited by 9
AI Summary: OriGene contributed to this study by providing a full-length, sequence-validated PRPF31 ORF clone with a C-terminal myc tag, which was used to express and characterize PRPF31 missense variants in the high-content imaging assay.
Snippet: Ciliopathies are a broad range of inherited developmental and degenerative diseases associated with structural or functional defects in motile or primary n.;Full-length, sequence-validated PRPF31 ORF clone with C-terminal myc tag was obtained from Origene. Singlenucleotide variants were introduced using NEB Q5 sitedirected mutagenesis kit. …
High-throughput PRPF31 variant characterisation pipeline consistent with ACMG/AMP clinical variant interpretation guidelines
Nazlamova, Cheung, Legebeke et al
medRxiv (2020)
Nazlamova, Cheung, Legebeke et al
medRxiv (2020)
Snippet: https://doi.org/10.1101/2020.04.06.20055020 doi: medRxiv preprint Page 8. 8 Full-length, sequence-validated PRPF31 ORF clone with C-terminal myc tag was obtained from 240 Origene. Single nucleotide variants were introduced using NEB Q5 site-directed mutagenesis;- https://doi.org/10.1101/2020.04.06.20055020 doi: medRxiv preprint Page 8. 8 Full-length, sequence-validated PRPF31 ORF clone with C-terminal myc tag was obtained from 240 Origene. …
A Combined in silico, in vitro and Clinical Approach to Characterize Novel Pathogenic Missense Variants in PRPF31 in Retinitis Pigmentosa
Wheway, Nazlamova, Meshad et al
Front Genet (2019) 10, 248 DOI: 10.3389/fgene.2019.00248
Wheway, Nazlamova, Meshad et al
Front Genet (2019) 10, 248 DOI: 10.3389/fgene.2019.00248
AI Summary: OriGene’s full-length, sequence-validated PRPF31 ORF clone with a C-terminal myc tag was used as the template for generating wild-type and mutant PRPF31 constructs, enabling in vitro functional studies in this research on retinitis pigmentosa.
Snippet: Variant Construct Cloning Full-length, sequence-validated PRPF31 ORF clone with C-terminal myc tag was obtained from OriGene. c.341T A or c.581C A variant was introduced using NEB Q5 site-directed mutagenesis kit. The entire wild-type and mutant clone sequence was verified by Sanger sequencing (Source Bioscience)
A combined in silico, in vitro and clinical approach to characterise novel pathogenic missense variants in PRPF31 in retinitis pigmentosa
Wheway, Nazlamova, Meshad et al
bioRxiv (2018)
Wheway, Nazlamova, Meshad et al
bioRxiv (2018)
Snippet: 121 Variant construct cloning 122 Full-length, sequence-validated PRPF31 ORF clone with C-terminal myc tag was obtained from 123 Origene. c.341T>A variant was introduced using NEB Q5 site-directed mutagenesis kit. The entire 124 View as HTML
Showing 1-4 of 4 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Related Products
You might also be interested by the following products:
Cat#
Description
Cond.
Price Bef. VAT