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Porcine IL-1 beta ELISA Kit
Porcine IL-1 beta ELISA Kit
RayBio® Porcine (pig) IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Porcine (pig) |
| Sensitivity | 6 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 6 pg/ml - 6000 pg/ml |
| Specificity | The sandwich ELISA antibody pair detects Porcine IL-1 beta. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P26889 |
| Gene Id | 397122 |
| Gene Symbols | IL1B |
| Protein Name / Synonyms | Interleukin-1 beta (IL-1 beta) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Porcine IL-1 beta qIPCR ELISA Kit
As low as $749.00
RayPlex® Porcine IL-1 beta Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Porcine IL-1 beta ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-1 beta (IL-1F2 / IL1B), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Platycodon grandiflorus Polysaccharide Attenuates Inflammation by Inhibiting NLRP3 Inflammasome Activation via the ROS/NEK7 Pathway
- Species:
- Porcine
- Sample Type:
- Cell Culture Supernatant (3D4/21 porcine alveolar macrophages)
- Year:
- 2026
- Journal Name:
- Molecules
- PubMed ID:
- 42451639
ASFV pA137R protein triggers inflammatory response by inducing NF-κB signaling pathway and facilitating NLRP3 inflammasome assembly
- Species:
- Porcine
- Sample Type:
- Cell Culture Supernatant (porcine alveolar macrophages, ASFV infection)
- Year:
- 2026
- Journal Name:
- Virologica Sinica
- PubMed ID:
- 42217584
Porcine Deltacoronavirus M Protein Binds NLRP3 to Promote Inflammasome Assembly via Competition with TRIM31
- Species:
- Porcine
- Sample Type:
- Cell Culture Supernatant; Serum; Feces / Gut Content (intestinal contents)
- Year:
- 2026
- Journal Name:
- Advanced science (Weinheim, Baden-Württemberg, Germany)
- PubMed ID:
- 42378650
African swine fever virus pB318L suppresses inflammatory response by inhibiting NF-κB activation and NLRP3 inflammasome formation
- Species:
- Porcine
- Sample Type:
- Serum
- Year:
- 2025
- Journal Name:
- Plos Pathogens
- PubMed ID:
- 41124215
The B169L protein of African swine fever virus functions as a viroporin that activates the calcium-mediated inflammasome
- Species:
- Porcine
- Sample Type:
- Cell Culture Supernatant (PAMs ASFV-infected; WSL cells)
- Year:
- 2025
- Journal Name:
- Plos Pathogens
- PubMed ID:
- 41237200
Can antioxidants' supplementation modulate the impact of dietary deoxynivalenol and zearalenone co-contamination on the systemic and mucosal immune response of weaned pigs?
- Species:
- Porcine
- Sample Type:
- Plasma (pooled per pen, d 7 and 14)
- Year:
- 2026
- Journal Name:
- Journal of Animal Science
- PubMed ID:
- 42114140
Effects of dietary medium-chain fatty acid supplementation levels on growth performance, fecal score, energy, antioxidant, inflammatory status, and fecal metabolites of nursery pigs fed high-protein diets
- Species:
- Porcine
- Sample Type:
- Serum (nursery pigs, MCFA diets)
- Year:
- 2026
- Journal Name:
- Journal of Animal Science
- PubMed ID:
- 42528260
Virus-like and Virus Replicon Particles Targeting Multiple B-Cell Antigens Do Not Protect Against African Swine Fever Virus
- Species:
- Porcine
- Sample Type:
- Serum (vaccinated, ASFV-challenged pigs)
- Year:
- 2026
- Journal Name:
- Vaccines
- PubMed ID:
- 41893821
The S273R protein of African swine fever virus antagonizes the canonical NF-κB signaling pathway by IκBα
- Species:
- Porcine
- Year:
- 2025
- Journal Name:
- Journal of Virology
- PubMed ID:
- 40162787
The African swine fever virus MGF300-4L protein is associated with viral pathogenicity by promoting the autophagic degradation of IKKβ and increasing the stability of IκBα
- Species:
- Porcine
- Sample Type:
- Cell Culture Supernatant (ASFV-infected PAMs, 12 and 20 hpi)
- Year:
- 2024
- Journal Name:
- Emerging microbes & infections
- PubMed ID:
- 38501350
