Porcine Cytokine Array Q3 (Glass Slide)

Cat# QAP-CYT-3-4

Size : 50SampleKit

Brand : RayBiotech

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Phone : +1 850 650 7790

Porcine Cytokine Array Q3

Quantibody® Porcine (pig) Cytokine Array 3 Kit. Detects 10 Porcine (pig) Cytokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size8 Sample Kit, 22 Sample Kit, 50 Sample Kit
SpeciesPorcine (pig)
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeQuantitative
Number of Targets Detected10
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice
Storage-20°C

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Decorin
  • Gene Symbol: DCN
  • Gene ID: 396957
  • Accession #: Q9XSD9
  • ELISA Kit: RayBio Porcine Decorin ELISA
GASP-1
(WFIKKNRP)
  • Gene Symbol: GPRASP1
  • ELISA Kit: RayBio Porcine GASP-1 ELISA
IGFBP-5
  • Gene Symbol: IGFBP5
  • Gene ID: 397182
  • Accession #: Q28985
  • ELISA Kit: RayBio Porcine IGFBP-5 ELISA
IL-15
  • Gene Symbol: IL15
  • Gene ID: 397683
  • Accession #: Q95253
IL-22
  • Gene Symbol: IL22
  • Gene ID: 595104
  • Accession #: K7GM74
  • ELISA Kit: RayBio Porcine IL-22 ELISA
Insulin
  • Gene Symbol: INS
  • Gene ID: 397415
  • Accession #: P01315
  • ELISA Kit: RayBio Porcine Insulin ELISA
IP-10
(CXCL10)
  • Gene Symbol: CXCL10
  • Accession #: Q2EN90
  • ELISA Kit: RayBio Porcine IP-10 ELISA
MCP-1
(CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 100135494
  • Accession #: Q08782
  • ELISA Kit: RayBio Porcine CCL2 ELISA
NCAM-1
(CD56)
  • Gene Symbol: NCAM1
  • ELISA Kit: RayBio Porcine NCAM-1 ELISA
TWEAK R
(TNFRSF12)
  • Gene Symbol: TNFRSF12A
  • Gene ID: 100217390
  • Accession #: B7SLY2
  • ELISA Kit: RayBio Porcine TWEAK R ELISA

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming Biological Process
  • Biomarker Validation
  • Validation of Antibody Array Results
  • Quantitative Protein Detection
  • Establishing Normal Range
Kit Components
  • Quantibody® Porcine Cytokine Array 3 Slide
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAP-CYT-3.gal)
Excel (QAP-CYT-3_Analysis_Tool.xls)

citations

  • Optimized Centrifugation and Activation Protocol for the Preparation of Plasma Rich in Growth Factors in Pigs

    Species:
    Porcine
    Sample Type:
    Cell Culture Supernatant (supernatants of PRGF activated with 13.3 mM CaCl2)
    Year:
    2026
    Journal Name:
    Biomedicines
    PubMed ID:
    41898286