Porcine Cytokine Array Q1 (Glass Slide)

Cat# QAP-CYT-1-1

Size : 8SampleKit

Brand : RayBiotech

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Phone : +1 850 650 7790

Porcine Cytokine Array Q1

Quantibody® Porcine (pig) Cytokine Array 1 Kit. Detects 10 Porcine (pig) Cytokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size8 Sample Kit, 22 Sample Kit, 50 Sample Kit
SpeciesPorcine (pig)
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeQuantitative
Number of Targets Detected10
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice
Storage-20°C

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF
  • Gene Symbol: CSF2
  • Accession #: Q29118
  • ELISA Kit: RayBio Porcine GM-CSF ELISA
IFN-gamma
  • Gene Symbol: IFNG
  • Accession #: P17803
  • ELISA Kit: RayBio Porcine IFN-gamma ELISA
IL-1 beta
(IL-1 F2)
  • Gene Symbol: IL1B
  • Accession #: P26889
  • ELISA Kit: RayBio Porcine IL-1 beta ELISA
IL-10
  • Gene Symbol: IL10
  • Accession #: Q29055
  • ELISA Kit: RayBio Porcine IL-10 ELISA
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: Q29053
  • ELISA Kit: RayBio Porcine IL-12 ELISA
IL-4
  • Gene Symbol: IL4
  • Accession #: Q04745
  • ELISA Kit: RayBio Porcine IL-4 ELISA
IL-6
  • Gene Symbol: IL6
  • Accession #: P26893
  • ELISA Kit: RayBio Porcine IL-6 ELISA
IL-8
(CXCL8)
  • Gene Symbol: IL8
  • Accession #: P26894
  • ELISA Kit: RayBio Porcine IL-8 ELISA
TGF beta 1
  • Gene Symbol: TGFB1
  • Accession #: P07200
  • ELISA Kit: RayBio Porcine TGF-beta-1 ELISA
TNF alpha
  • Gene Symbol: TNF
  • Accession #: P23563
  • ELISA Kit: RayBio Porcine TNF-alpha ELISA

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Porcine Cytokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAP-CYT-1.gal)
Excel (QAP-CYT-1_Analysis_Tool.xls)

citations

  • Ningxiang pig-derived Enterococcus hirae protects against E. coli-induced gut dysbiosis and inflammation via acetate/propionate-MyD88-NF-κB axis in piglets

    Species:
    Porcine
    Sample Type:
    Other / Unspecified (piglets ileal mucosa)
    Year:
    2026
    Journal Name:
    Microbiome
    PubMed ID:
    41491532
  • Optimized Centrifugation and Activation Protocol for the Preparation of Plasma Rich in Growth Factors in Pigs

    Species:
    Porcine
    Sample Type:
    Cell Culture Supernatant (supernatants of PRGF activated with 13.3 mM CaCl2)
    Year:
    2026
    Journal Name:
    Biomedicines
    PubMed ID:
    41898286
  • An improved porcine model of infrarenal abdominal aortic aneurysm

    Year:
    2025
    Journal Name:
    Scientific Reports
    PubMed ID:
    41408131
  • Alginate-Encapsulated Mesenchymal Stromal Cells Improve Hind Limb Ischemia in a Translational Swine Model

    Species:
    Porcine
    Sample Type:
    Serum (frozen serum samples)
    Year:
    2024
    Journal Name:
    Journal of the American Heart Association
    PubMed ID:
    38639336
  • Ghrelin modulates innate immune and ileal responses to lipopolysaccharide administration in weaned pigs

    Species:
    Porcine
    Sample Type:
    Serum (LPS-challenged weaned pigs, time course)
    Year:
    2025
    Journal Name:
    Frontiers in Animal Science
  • Ningxiang pigderived Enterococcus hirae regulates the inflammatory function and enhances the protection of piglets against ETEC challenge

    Year:
    2024
    Journal Name:
    Frontiers in Cellular and Infection Microbiology
    PubMed ID:
    39483124
  • Dietary taurine supplementation counteracts deoxynivalenol-induced liver injury via alleviating oxidative stress, mitochondrial dysfunction, apoptosis, and inflammation in piglets

    Species:
    Pig
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Ecotoxicology and Environmental Safety
  • Characterization of serum proteomic and inflammatory profiling at early stage of iron deficiency in weaned piglets

    Species:
    Porcine
    Sample Type:
    Serum (iron-deficient weaned piglets)
    Year:
    2024
    Journal Name:
    Animal nutrition (Zhongguo xu mu shou yi xue hui)
    PubMed ID:
    39296492
  • Parabacteroides distasonis ameliorates ETEC-induced diarrhea in weaned piglets by regulating intestinal barrier function and intestinal inflammation

    Year:
    2026
    Journal Name:
    Porcine Health Management
    PubMed ID:
    42316269
  • The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response

    Species:
    Porcine
    Sample Type:
    Serum (serum-free media from IPEC-J2 cultures); Tissue Lysate / Homogenate (mouse intestinal tissue homogenate)
    Year:
    2021
    Journal Name:
    Nucleic Acids Research
    PubMed ID:
    33999206

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