RayBio® C-Series Porcine Cytokine Array C1 Kit. Detects 48 Porcine Cytokines. Suitable for all liquid sample types.
Porcine Cytokine Array C1
Porcine Cytokine Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Porcine (pig) |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Accession Number | Q9TU73|A1YM34|Q711P4|B0FYK2|Q9XSD9|Q29118|P49879|Q29412|P17803|Q28985|Q29053|Q95J68|Q95253|Q60I29|F1RKV0|P18430|P26889|Q29056|K7GM74|D0FHA1|Q04745|P26893|P26894|P01315|P80928|F1SM72|F1S278|P20034|K7GPI3|Q29288|Q29030|P35624|C0JPM4|P23563|B7SLY2|P49151|P49157|F1S1F8|C6JWG4|P23695|Q29055|Q76LU6|Q58IV6|P07200 |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 48 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
| Angiopoietin-1
| CCL3L1
| Decorin
| Eotaxin-1 (CCL11)
| |
| Galectin-9
| GASP-1 (WFIKKNRP)
| GM-CSF
| IFN alpha
| |
| IFN-gamma
| IGFBP-5
| IL-1 alpha (IL-1 F1)
| ||
| IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3)
| IL-12 p70
| IL-13
| |
| IL-15
| IL-17A
| IL-17F
| IL-18
| |
| IL-22
| IL-4
| IL-6
| IL-8 (CXCL8)
| |
| Insulin
| MIF
| MIG (CXCL9)
| MIP-1 beta (CCL4)
| NCAM-1 (CD56)
|
| Osteoprotegerin (TNFRSF11B)
| PDGF-BB
| PECAM-1 (CD31)
| RANTES (CCL5)
| |
| TGF alpha
| TIMP-1
| TIMP-2
| ||
| TNF alpha
| TWEAK R (TNFRSF12)
| VEGF-A
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Porcine Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Prediction of Patient Drug Response via 3D Bioprinted Gastric Cancer Model Utilized Patient-Derived Tissue Laden Tissue-Specific Bioink
- Species:
- Porcine
- Sample Type:
- Other / Unspecified (protein extracts of g-dECM, matrigel)
- Year:
- 2025
- Journal Name:
- Advanced science (Weinheim, Baden-Württemberg, Germany)
- PubMed ID:
- 39748450
The impact of cooling and Moringa supplementation on oxidative stress in serum and milk, including milk cytokines, in heat stressed lactating sows and their litters
- Species:
- Porcine
- Sample Type:
- Other Body Fluid (milk whey)
- Year:
- 2025
- Journal Name:
- Translational animal science
- PubMed ID:
- 39844792
Effects of mesenchymal stromal cells and human recombinant Nerve Growth Factor delivered by bioengineered human corneal lenticule on an innovative model of diabetic retinopathy
- Species:
- Porcine
- Sample Type:
- Cell Culture Supernatant (conditioned media from HG-treated neuroretinal explants)
- Year:
- 2024
- Journal Name:
- Frontiers in Endocrinology
- PubMed ID:
- 39473506
Increased efficiency of peripheral nerve regeneration using supercritical carbon dioxide-based decellularization in acellular nerve graft
- Year:
- 2024
- Journal Name:
- Scientific Reports
- PubMed ID:
- 39389997
Minimally invasive delivery of therapeutic agents by hydrogel injection into the pericardial cavity for cardiac repair
- Species:
- Porcine
- Sample Type:
- Other Body Fluid (pericardial fluid)
- Year:
- 2021
- Journal Name:
- Nature Communications
- PubMed ID:
- 33658506
Dietary Synbiotic Attenuated the Intestinal Inflammation in Weaned Piglets Challenged with Escherichia coli Lipopolysaccharide
- Species:
- Porcine
- Sample Type:
- Other / Unspecified (jejunum, colon)
- Year:
- 2025
- Journal Name:
- Animals
- PubMed ID:
- 40646731
Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization
- Species:
- Rat
- Sample Type:
- Schwann cell
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs
- Species:
- Porcine
- Year:
- 2020
Matrix Bound Nanovesicles Have Tissue-Specific Characteristics That Suggest a Regulatory Role
- Species:
- Porcine
- Sample Type:
- extracellular vesicle (EV)
- Year:
- 2022
- Journal Name:
- Tissue Engineering Part A
