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Reagents and instruments for immunology, cell biology and molecular biology.
Quimigen Portugal
> pLenti-P2A-tGFP Lentiviral Gene Expression Vector
pLenti-P2A-tGFP Lentiviral Gene Expression Vector
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Quantity :
Cat# PS100108
Size :
10ug
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pLenti-P2A-tGFP Lentiviral Gene Expression Vector
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SKU
PS100108
pLenti-P2A-tGFP, tag free Lenti vector with P2A-tGFP, 10 ug
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Specifications
Product Data
E. coli Selection
Chloramphenicol (34 ug/mL)
Mammalian Cell Selection
tGFP
Expression Promoter
CMV
Features
This Lenti vector is a third generation lentiviral vector.
All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
The ORF (no stop codon for P2A-tGFP) cloned in this vector will be expressed as native protein, no tag.
P2A-tGFP is within the Lentiviral packaging cassette and would allow GFP to enrich both the transfected and the transduced cells.
A brief lentiviral packaging protocol is provided.
E. Coli.
selection: Chloramphenicol (34 ug/ml)
V2_F Primer can be used to sequence the target sequence after cloning.
AC_GFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping
Ambient
Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS for PS100108
Vector Resources
Video Tutorial: Clone Selection Guide
Product Video: Why Should You Choose TrueORF GOLD
shRNA Selection Guide
Protocol: Lentivirus packaging
CRISPR Knockout kit manual
CRISPR vector manual
CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual
CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual
AAV Biosafety
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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