PICP ELISA Kit (Rat)

Cat# OKEH03702

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for PICP ELISA Kit (Rat) (OKEH03702)
Product Info
Predicted Species ReactivityRat
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PICP ELISA Kit (Rat) (OKEH03702) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PICP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for PICP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PICP captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Component
ComponentAmount
Anti-PICP Microplate96 Wells (12 x 8 Well strips)
PICP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X PICP-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.437 ng/mL
SpecificityNatural and recombinant Rat C-terminal propeptide of Collagen alpha-1(I) chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPICP
Gene Full NameC-terminal propeptide of Collagen alpha-1(I) chain
Alias SymbolsC-terminal propeptide of Collagen alpha-1(I) chain
Protein NameC-terminal propeptide of Collagen alpha-1(I) chain

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Price Bef. VAT
OKEH03703
 96Wells