phospho-LRP5 (Thr1492) Polyclonal Antibody
Cat# bs-25114R
Size : 100uL
Brand : Bioss
Contact local distributor :
Phone : +1 850 650 7790
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-LRP5 (Thr1492)) Polyclonal Antibody, Unconjugated (bs-25114R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-25114r |
| Product Name | phospho-LRP5 (Thr1492) Polyclonal Antibody |
| Applications | IHC-P, IHC-F, IF |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human LRP5 around the phosphorylation site of Thr1492 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4041 |
| Swiss Prot | O75197 |
| Background | Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation. |
| Application Dilution | |
| IHC-P | =1:100-500 |
| IHC-F | IHC-F=1:400-800 |
| IF | IF=1:100-500 |
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