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Dispatch Time: About 3 working days
- Product Name
- PerCP-Cy5.5 Anti-Mouse CD40 Antibody(FGK4.5/FGK45)
- Catalogue No.
- PCP55-30021
- Form
- liquid
- Conjugation
- PerCP-Cy5.5
- Conjugation Information
- PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).
- Clonality
- Monoclonal
- Isotype
- IgG2a, κ
- Clone ID
- FGK4.5/FGK45
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- B-cell surface antigen CD40|Bp50|CD40|CD40L receptor|Cd40|Tnfrsf5|Tumor necrosis factor receptor superfamily member 5 antibody
- UniProt ID
- P27512
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
Staining of C57BL/6 murine splenocytes with FITC Anti-Mouse CD19 Antibody(1D3) and PerCP-Cy5.5 Anti-Mouse CD40 Antibody(FGK4.5/FGK45) (left) or PerCP-Cy5.5 Rat IgG2a, κ Isotype Control (right). Total viable cells were used for analysis. - Background
- CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.