Quantibody® Ovine (sheep) Cytokine Array 1 Kit. Detects 10 Ovine Cytokines. Suitable for all liquid sample types.
Ovine Cytokine Array Q1
Ovine Cytokine Array Q1
Contact Us for Bulk Request
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Ovine (sheep) |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Decorin
| IFN-gamma
| IL-17A
| IL-21
| IL-8
|
IP-10 (CXCL10)
| MIG (CXCL9)
| sFRP-3
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Ovine Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAO-CYT-1.gal)
Excel (QAO-CYT-1_Analysis_Tool.xls)
citations
Filifactor alocis FtxA blocks inflammation and apoptosis pathways in monocytic cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (THP-1 cell supernatants)
- Year:
- 2026
- Journal Name:
- Frontiers in Cellular and Infection Microbiology
- PubMed ID:
- 41947787
Immunological and Pathogenic Differences of Two Experimental Bluetongue Virus Serotype Infections Evaluated in Two Disparate Host Species
- Year:
- 2024
- Journal Name:
- Viruses
- PubMed ID:
- 39459926
Is Umbilical Cord Blood Therapy an Effective Treatment for Early Lung Injury in Growth Restriction?
- Species:
- Sheep
- Sample Type:
- Tissue Lysate / Homogenate (lung tissue lysate)
- Year:
- 2020
- Journal Name:
- Frontiers in Endocrinology
- PubMed ID:
- 32194502
Monitoring longitudinal immunological responses to bluetongue virus 17 in experimentally infected sheep
- Species:
- Sheep
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Virus Research