NODAL Protein Lysate
Cat# OCOA02393
Size : 20µg
Brand : Aviva Systems Biology
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NODAL Protein Lysate (OCOA02393)
| Datasheets/Manuals | Printable datasheet for NODAL Protein Lysate (OCOA02393) |
|---|
| Predicted Species Reactivity | Homo sapiens |
|---|---|
| Product Format | Lyophilized |
| Host | HEK293T |
| Additional Information | Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| :: | Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution and Storage | 2°C to 8°C |
| Tag | C-MYC/DDK |
| Gene Symbol | NODAL |
|---|---|
| Gene Full Name | nodal growth differentiation factor |
| Alias Symbols | HTX5, nodal homolog. |
| NCBI Gene Id | 4838 |
| Protein Name | Homo sapiens nodal growth differentiation factor (NODAL), transcript variant 1, mRNA|Nodal homolog|nodal homolog isoform 1 preproprotein [Homo sapiens] |
| Description of Target | Essential for mesoderm formation and axial patterning during embryonic development. |
| Uniprot ID | Q96S42 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_060525 |
| Nucleotide Accession # | https://www.ncbi.nlm.nih.gov/nuccore/NM_018055 |
| Molecular Weight | 39.58 kDa |