Nitrotyrosine Antibody
Cat# OASE00079
Size : 100ug
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Nitrotyrosine Antibody (OASE00079)
| Datasheets/Manuals | Printable datasheet for Nitrotyrosine Antibody (OASE00079) |
|---|---|
| COA Datasheet | 0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Predicted Species Reactivity | All Species|Non-species Specific |
|---|---|
| Product Format | Liquid PBS with 50% glycerol and 0.09% sodium azide |
| Clonality | Monoclonal |
| Clone | 39B6 |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | Unconjugated |
| Application | AM,ELISA,FC,ICC,IF,IHC,IP,WB |
| :: | Certificate of Analysis: 0.7ug/ml was sufficient for detection of 5ug SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Reconstitution and Storage | -20°C |
| Immunogen | 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin |
| Purification | Protein G Purified |
| Concentration | 1 mg/ml |
| Specificity | Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific. |
| Dilution | WB (1:1400), IHC (1:100); optimal dilutions for assays should be determined by the user. |
| Description | Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4). |
| Reference | 1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. 3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330(2): 795-801. |
|---|---|
| Gene Symbol | Nitrotyrosine |
| Alias Symbols | Nitro tyrosine, 3-Nitrotyrosine |
| Description of Target | Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. |
