NeoPRO RTU Femto - Ready to Use Western ECL Substrate
Cat# NB-78-00009
Size : 125mLkit
Brand : Neo Biotech
Contact local distributor :
Phone : +1 850 650 7790
Description
NeoPRO RTU Femto from Neo Biotech is a patented, next-generation chemiluminescent substrate developed for researchers who demand the highest levels of sensitivity in Western blot detection. Its innovative single-solution formulation delivers low-femtogram detection sensitivity, making it the most sensitive ready-to-use ECL substrate in its category.
Designed to simplify workflows while maximizing analytical performance, NeoPRO RTU Femto eliminates reagent mixing, reducing preparation time and minimizing potential experimental variability. Bring the reagent to room temperature, and it is ready for use.
Its superior signal intensity enables detection of extremely low protein concentrations that may remain undetectable with conventional chemiluminescent substrates, providing greater confidence when working with low-abundance proteins, limited sample quantities, or challenging biological targets.
Benchmarked against: Immobilon® Forte under identical experimental conditions.
Key Performance Data at a Glance
| Metric | NeoPRO RTU Femto Performance |
|---|---|
| Detection level | Low-femtogram. |
| Format | Patented, ready-to-use, single-solution formulation; no mixing required. |
| Detectable bands vs. Immobilon® Forte under the same conditions | 6 bands vs. 3 bands. |
| Signal duration | Up to 2 hours. |
| Shelf life | 1 year at 2–8 °C. |
| Shipping | Room temperature. |
Why Choose NeoPRO RTU Femto?
Exceptional Sensitivity for Challenging Targets
Under identical experimental conditions, NeoPRO RTU Femto demonstrated superior detection performance compared with Immobilon® Forte, revealing six detectable bands versus three and highlighting its sensitivity and signal-generation capability.
High Performance with Lower Antibody Consumption
The substrate’s efficiency enables significantly higher antibody dilutions while maintaining strong signal intensity, reducing reagent consumption and experimental costs while improving laboratory productivity.
Patented One-Solution Technology
Its patented one-solution technology eliminates reagent mixing, reducing hands-on time and workflow complexity while maximizing convenience and reproducibility.
Ready to Use After Reaching Room Temperature
Bring NeoPRO RTU Femto to room temperature, and it is ready for use. No additional preparation steps are required, reducing experimental variability.
Product Properties
NeoPRO RTU Femto — Ultra-Sensitive, Ready-to-Use Low-Femtogram ECL Substrate
| Property | Details |
|---|---|
| Features | Low-femtogram detection. No mixing required. Low antibody consumption to reduce costs. Ready to use after reaching room temperature. |
| Substrate type | HRP (horseradish peroxidase) substrate. |
| Components | Luminol derivative/enhancer/peroxide solution. |
| Antibody dilution range | Primary: 1:5,000–1:100,000. Secondary: 1:100,000–1:500,000. (From 1 mg/mL stock solutions.) |
| Available format | 250 mL kit (2,500 cm2 of membrane). |
| Signal duration | 2 hours. |
| Storage | Stable for 1 year at 2–8 °C. |
| Shipping | Room temperature. |