NeoPRO Atto Western ECL Substrate
Cat# NB-78-00006-10
Size : 2x5ml
Brand : Neo Biotech
Contact local distributor :
Phone : +1 850 650 7790
Description
NeoPRO Atto Western ECL Substrate is Neo Biotech’s most powerful chemiluminescent (ECL) substrate. Based on exclusive patented technology, it achieves attogram-level protein detection and outperforms leading premium substrates in the reported comparisons.
Benchmark substrates: NeoPRO Femto PLUS, SuperSignal™ West Femto (Thermo Scientific™), Amersham™ ECL Select™ (GE Healthcare), and Clarity Max™ (Bio-Rad).
Key Performance Data at a Glance
| Metric | NeoPRO Atto Western ECL Substrate Performance |
|---|---|
| Detection level | Attogram. |
| Linear dynamic range (R²) | > 0.99. |
| Signal-to-noise ratio vs. leading competitors | Up to 10-fold higher. |
| Protein sample required vs. ECL Select, equal signal | One-eighth of the protein amount. |
| Primary antibody required vs. Clarity Max, equal signal | One-tenth of the primary antibody amount. |
| Exposure time vs. Clarity Max, equal signal | 10 seconds vs. 5 minutes. |
| Reproducibility in triplicate | < 20% variability. |
| Signal duration | 5 hours. |
Why Choose NeoPRO Atto Western ECL Substrate?
Superior Performance vs. Leading Substrates
NeoPRO Atto Western ECL Substrate exhibits superior performance in the reported comparisons with NeoPRO Femto PLUS, SuperSignal™ West Femto, Amersham™ ECL Select™, and Clarity Max™.
Extreme Sensitivity and Wide Linear Range
With R² > 0.99, it offers a wide linear dynamic range and a signal-to-noise ratio up to 10-fold higher than the leading ECL substrates tested, supporting accurate interpretation of even faint bands.
Save Precious Samples and Antibodies
Its high signal intensity enables equivalent signal with one-eighth of the protein amount required with Amersham™ ECL Select™, and comparable performance with one-tenth of the primary antibody amount used with Clarity Max™ in the reported experiments.
Shorten Exposure Time
It delivers signal intensity and sensitivity comparable to Clarity Max™ with a 10-second exposure instead of 5 minutes in the reported comparison, saving time and improving productivity.
High Reproducibility
In a simplified multiwell assay, substrate performance is assessed independently of sample loading, transfer efficiency, blocking, and antibody performance. Reported variability in triplicate is below 20%.
Product Properties
NeoPRO Atto Western ECL Substrate — Ultra-Sensitive Attogram-Level for Western Blot
| Property | Details |
|---|---|
| Description | Exclusive patented technology for attogram-level protein detection. Extreme sensitivity and strong light output, with low background and an extremely high signal-to-noise ratio. |
| Features | Attogram-level protein detection. Improved interpretation of faint bands. Short exposure times. Reduced consumption of antibodies and precious samples. |
| Components | Luminol derivative/enhancer solution (A). Peroxide solution (B). |
| Antibody dilution range | Primary: 1:10,000–1:200,000. Secondary: 1:300,000–1:1,000,000. (From 1 mg/mL stock solutions.) |
| Available formats | 2 × 10 mL kit: 200 cm2 of membrane. 2 × 50 mL kit: 1,000 cm2 of membrane. |
| Signal duration | 5 hours. |
| Storage and shipping | Stable for 1 year at room temperature. Shipped at ambient temperature. |